Figure 5.

Combined pharmacological inhibition of HH-GLI and ERK5 pathways synergistically reduces melanoma cell proliferation. Colony formation assays were performed in SSM2c (A), MeWo (B), and A375 (C) untreated (NT) cells or cells treated with DMSO (Vehicle), JWG-071 (1 μM), or GANT61 (1 μM), alone or in combination (JWG + GANT). Histograms represent mean ± SD from three independent experiments. Representative images of plates are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as determined by Student’s t-test. ^§ Bliss independence score (>0) indicates synergistic effects over single treatments. (D) Cell viability assay was performed on SSM2c, MeWo, or A375 cells treated with DMSO (Vehicle) or with the indicated treatment for 72 h. MTT assay was performed in three independent experiments. In the graph are indicated the bliss independence scores for all drug combinations. Bliss score > 0 indicates synergism, = 0 additivity, < 0 antagonism.