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. 2021 Sep 29;13(10):1582. doi: 10.3390/pharmaceutics13101582

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Secretions of IgG (a), isotypes IgG1 (b), and IgG2a (c) in the sera separated from immunized animals at week zero, two, and four. The dilution ratio of tested sera was 1:100 in ELISA analysis, and each serum was investigated once. Values were analyzed by one-way ANOVA analysis followed by Dunnett’s test. Values between the pVAX1-SIR2/CS and pVAX1-SIR2/PLGA group were estimated by the independent t-test. Values were shown as mean of OD450 ± SD (n = 5). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with blank or control group.