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. 2021 Sep 29;13(10):1582. doi: 10.3390/pharmaceutics13101582

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of MHC molecules on the surface of DCs at week zero, two, and four. The expression of MHC-I and MHC-II molecules was evaluated by flow cytometry. Bar graph showed the ratio of MHC-I (a) and MHC-II (b) molecules on splenic DCs, and the dot plots (c) showed the percentage of CD11c⁺MHC-I⁺ and CD11c⁺MHC-II⁺ cells. Each sample was investigated once, and values were analyzed by one-way ANOVA analysis followed by Dunnett’s test. Values between the pVAX1-SIR2/CS and pVAX1-SIR2/PLGA group were estimated by the independent t-test. Values were shown as mean ± SD (n = 5). ** p < 0.01 and *** p < 0.001 compared with blank or control group.