Figure 9.

Splenocyte proliferation of immunized animals. Three mice from each group were euthanized, the spleen lymphocytes were collected, and the lymphocytes from one mouse were randomized into four replications. Then, the lymphocytes were cultured with 20 μg/mL rTgSIR2 protein. Each replication was investigated once, and values were analyzed by one-way ANOVA analysis followed by Dunnett’s test. Values between the pVAX1-SIR2/CS and pVAX1-SIR2/PLGA group were estimated by the independent t-test. Values were shown as mean ± SD (n = 3). ** p < 0.01 and *** p < 0.001 compared with blank or control group.