Figure 4.

The spike proteins induce a decrease in the mitochondrial protein in the brain ECs. Primary human brain ECs were treated with 15 nM of S1 domain of spike protein (S1) and active trimer (Trimer) for 15, 30 min, and 2 h, respectively. Both are stained with antibody against translocase of the outer mitochondrial membrane complex subunit (TOMM) 20, a marker for the mitochondria (green). DAPI (blue) indicates nucleus. Insets indicate magnified images of squares of dashed lines. Scale bar, 75 μm (left panel); 20 μm (right panel).