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. 2021 Oct 15;26(20):6230. doi: 10.3390/molecules26206230

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of glycosyltransferase inhibitor effect using bioluminescent nucleotide assays. (a) Inhibition of OGT by two compounds detected with UDP-Glo assay. (b) Inhibition of FUT7 Gallic acid detected with GDP-Glo assay. To control for assay reagent inhibition with the compounds, a titration of the compounds was performed in the presence of the nucleotide with no enzyme. Curve fitting and IC₅₀ value determinations were performed using GraphPad Prism^® version 9, sigmoidal dose-response (variable slope) software. Reactions were performed in duplicates, and the results shown are means ± standard deviations.