Table 2.
Trisaccharide proportions produced from 500 mM or 1500 mM sucrose by the BaAG2. The sugar in each trisaccharide in bold was used for integration. The sucrose moiety in trisaccharides is underlined.
| Saccharide | Composition and Linkage Type |
500 mM Sucrose | 1500 mM Sucrose |
|---|---|---|---|
| Isomelezitose * | Glc-α1,6-Fru-β2,1-Glc | 32.2% | 25.0% |
| Erlose | Glc-α1,4-Glc-α1,2-Fru | 25.8% | 31.7% |
| Theanderose * | Glc-α1,6-Glc-α1,2-Fru | 22.7% | 20.5% |
| Melezitose | Glc-α1,3-Fru-β2,1-Glc | 9.6% | 15.9% |
| 1-OF-Glucosyl-Sucrose | Glc-α1,1-Fru-β2,1-Glc | 3.7% | 2.7% |
| Esculose | Glc-α1,3-Glc-α1,2-Fru | 2.6% | 0.8% |
| 4-OG-Glucosyl-Turanose | Glc-α1,4-Glc-α1,3-Fru | 2.0% | 1.7% |
| 4-OF-Glucosyl-Sucrose | Glc-α1,4-Fru-β2,1-Glc | 1.4% | 1.7% |
* As the anomeric signals overlapped, the relative ratio of the two were determined using the signal arising from the 6-position of the 6-substituted Glc in theanderose and the 6-position of the 2,6-substituted Fru in isomelezitose.