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. 2021 Oct 23;809:151137. doi: 10.1016/j.scitotenv.2021.151137

Table 4.

Summary of the methodology used and of the results found in different datasets focused on detecting SARS-CoV-2 genetic material (RNA), in different community indoor sites.

Reference Sites Sampling Method Results for air samples Notes
Conte et al. (2021)
Italy
2 supermarket, 1 train station, 1 canteen, 1 commercial centre, 1 pharmacy, 1 hair salon in different cities in north, central, and south Italy. PM10 sampling at 38.3 L min−1 on quartz fibre filters, volumes 22.9–29.7 m3.
TSP sampling at 13.4 L min−1 and at 61.7 L min−1 on quartz filters, volumes 6.2–9.3 m3.
RT-PCR targeting RdRp, N, E genes.
dd-PCR targeting RdRp and RNAse P genes.
All of 56 PM10 samples tested negative.
All of 13 TSP samples tested negative.
LOD 1.3 copies m−3 for PM10 and 4 copies m−3 for TSP.
Recovery 54%.
de Rooij et al. (2021a)
Netherlands
Measurements taken inside different mink farms Sampling of inhalable dust (at 3.5 L min−1) on Teflon filters. Volume 1.26 m3. RT-qPCR targeting E gene. 3/27 air samples positive.
Conc. 2400–4900 copies m−3.
LOD 10–28 copies m−3.
Recovery not reported.
de Rooij et al. (2021b)
Netherlands
Measurements in a meat processing plant experiencing COVID-19 clusters. Inhalable dust sampled on Teflon filters at 3.5 L min−1. Volume 1.266 m3. RT-qPCR targeting RdRp and E genes. All of 14 air samples tested negative. LOD 3.2 copies/reaction. Recovery not reported.
6/203 surface samples positive.
Di Carlo et al. (2020)
Italy
A bus operating in Chieti (central Italy) in May 2020. Sampler (AMS Analitica) on gelatine filters at 24 L min−1. Volume 18.72 m3. RT-PCR targeting ORF1ab, N, S genes. All of 14 air samples tested negative. LOD and recovery not reported.
0/90 surface samples positive.
Habibi et al. (2021)
Kuwait
Samples in different indoors at the Kuwait Institute of Research. Sampling at 30 L min-1 in wash bottles with TRIzol (APB Bioscience). Volume 3.6 m3. RT-qPCR targeting ORF1ab, N genes. All of 5 air samples tested negative. Recovery not reported.
Hadei et al. (2021)
Iran
Public sites in Theran: 3 banks, shopping centres, post office and a governmental building, airport, subway station and train, bus. One TSP AV100 sampler at 40 L min-1 on PTFE filters, volumes 1.27–3.5 m3. A SKC pump at 3.5 L min−1 for bus and train sampling, volumes 0.2–0.24 m3. RT-PCR targeting ORF1ab and N genes. 18/28 air samples positive. Recovery 20%.
Moreno et al. (2021)
Spain
Subway trains and buses in Barcelona. PM2.5 sampler at 10 L min−1 on Teflon filters. Volumes 5.2–6.3 m3. RT-qPCR targeting RNA polymerase (IP2, IP4) and N genes. 2/6 subway air samples positive. Conc. 18.8–23.4 copies m−3.
1/6 bus air samples positive. Conc. 1.44 copies m−3.
6/15 surface samples in subway positive.
13/30 surface samples in buses positive.
Viegas et al. (2021)
Portugal
Ten higher education facility in Lisbon area. Coriolis μ sampler at 300 L min−1 on 5 mL vial with buffer NVL. Volume 0.6 m3. RT-PCR not reported the targets. All of 48 air samples tested negative. LOD and recovery not reported.
0/106 surface sample positive.
Xie et al. (2020)
China
2 houses with confirmed Covid-19 patients in a residential building in Guangzhou. Not specified details of the sampling approach used. RT-PCR not clear the target genes. All of 3 air samples tested negative. LOD and recovery not reported.
1/31 surface sample positive (door handle).
Yamagishi et al. (2020)
Japan
7 cabins of the Diamond Princess cruise ship. 2 MD8 samplers at 50 L min-1 on gelatine filters. Volume 1 m3. rRT-PCR not clear the target genes. All of 14 air samples tested negative. LOD and recovery not reported.
58/587 surface samples positive.