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Kinetics of [α-32P]UTP and [α-32P]CTP incorporation into mitochondrial RNA. Mitochondrial vesicles were labeled with [α-32P]UTP (U) or [α-32P]CTP (C) for the indicated times. Total RNA (total) and poly(A)+ RNA (A+) were isolated, and incorporation of radioisotope was measured by scintillation counting. The maximal amount of labeled nucleotide incorporation observed for each sample was designated 100% incorporation.
