REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-HCP	Antibodies-online.com	RRID: AB_1113182
anti-rabbit IgG HRP	Sigma	Cat# A9169; RRID:AB_258434
equine F(ab’)2 fragment	This paper	N/A
Horseradish peroxidase-conjugated rabbit anti-horse IgG	Sigma, USA	Cat# A6917; RRID:AB_258319
Bacterial and virus strains
SARS-CoV-2 isolate (SARS-CoV-2/human/BRA/RJ01/2020) - IRB approval 30650420.4.1001.0008		GenBank #MT710714
SARS-CoV-2 isolate from the B lineage (HIAE-02: SARS-CoV-2/SP02/human/2020/BRA)	Dr. Edison L. Durigon (Laboratory of Clinical and Molecular Virology, Institute of Biomedical Sciences, University of São Paulo)	GenBank #MT126808.1
P.1 strain (EPI_ISL_1060902 – GISAID)	Dr. Lucy S. Villas-Boas and Prof .Maria Cassia Mendes-Correa (Laboratory of Medical Investigation in Virology from University of Sao Paulo) - Faria et al. (2021)	EPI_ISL_1060902 – GISAID
P.2 variant	Voloch et al. (2021)	N/A
SARS-CoV-2/B.1.33 variant	hCoV-19/Brazil/RJ-MD63-1a/202	Accession ID: EPI_ISL_528637
Biological samples
Serum of convalescent patients	Alvim et al., (2020)	Sample code numbers: #8942, #1639, #3065
Chemicals, peptides, and recombinant proteins
Recombinant SARS-CoV-2 spike protein	Alvim et al., (2020)	N/A
HEK-GM medium	Xell AG, Germany	N/A
Superose 6 10/300 GL column	Cytiva	#29091596
MW standards standardized molecules	Bio-rad,	#151-1901
Rapigest SF	(Waters, Milford, MA)	N/A
PNGase F	New England BioLabs	#P0704S
Montanide ISA 50V adjuvant	Seppic, France	N/A
Pepsin	Sigma, USA	N/A
Tween 20	Sigma, USA	N/A
OPD substrate	Sigma, USA	N/A
fetal bovine serum	HyClone/Cytiva, USA	N/A
Streptomycin	Thermo Fisher, USA	N/A
ketamine hydrochloride	Vetanarcol, König, Argentina	N/A
xylazine hydrochloride	Syntec Brazil, São Paulo, Brazil	N/A
sodium thiopental	Thiopentax, Cristalia, São Paulo, Brazil	N/A
Chemagic Viral DNA/RNA 300 kit H96	Perkin-Elmer, Waltham, USA	N/A
RT-qPCR - Bio-Manguinhos SARS-CoV-2 molecular kit	Bio-Manguinhos, Rio de Janeiro, Brazil	N/A
Experimental models: Cell lines
African green monkey kidney (Vero, subtype E6)	ATCC	CRL-1586
HEK293-COV2-S	Alvim et al. (2020)	N/A
Experimental models: Organisms/strains
Female BALB/c mice, 6–8 weeks old	Central Bioterium (Centro de Ciências da Saúde – Universidade Federal do Rio de Janeiro, Brazil)	N/A
Golden Syrian hamsters Mesocricetus auratus, males and females, age between 1.5–2.0 years	Institute of Science and Technology in Biomodels (ICTB) of the Oswaldo Cruz Foundation (Fiocruz)	N/A
Ten 3- to 5-year-old healthy horses (6 males and 4 females) approximately 350 kg each	IVB farm, Rio de Janeiro, Brazil	N/A
Software and algorithms
GraphPad Prism 7®	GraphPad Software	N/A
GraphPad Prism® software (8.01 version)	GraphPad Software	N/A