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Western blotting of recombinant Drk1 and P. brasiliensis proteins recognized by anti-Drk1 antibody. Western blotting of (A) recombinant and (B) native Drk1 of P. brasiliensis. The non-specific reaction was evaluated using a pre-immune serum. M: standard protein marker (ColorBurstTM Sigma-Aldrich, Saint Louis, MO, USA; 8–220 kDa). The molecular mass is indicated on the left in kilodaltons (kDa).
