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. 2021 Oct 8;13(10):2032. doi: 10.3390/v13102032

Figure 1.

Figure 1

Antiviral activity of GmRUN1. (A) Gene architecture of GmRUN1. Block and line indicate exon and intron, respectively. (B) Evolutionary analyses of GmRUN1. The evolutionary tree was built using the neighbor-joining method conducted in MEGA7. All positions with less than 50% site coverage were eliminated. (C) Transient expression assay for GmRUN1 antiviral activity for TMV. N. benthamiana leaves were infiltrated with Agrobacterium tumefaciens GV3101 inocula (OD600 = 1.0) carrying different recombinant vectors and co-infected with TMV-GFP. GFP was visualized under hand-held UV lamp (Wavelength = 365 nm) at 5 dpi (days post infiltration). N: tobacco N protein. SRC7: SMV resistance cluster 7. EV: empty vector. GmRUN1 cDNA-1: GmRUN1 cDNA expressed from pCB301 vector. GmRUN1 cDNA-2: GmRUN1 cDNA expressed from pBI211 vector. (D) Fluorescence quantification of GmRUN1 transient expression assay. TMV-GFP intensity was analyzed by Gel-Pro analyzer software and normalized against positive control (N). (E) Transient expression assay for GmRUN1 antiviral activity for SMV.