Figure 2.

Truncation analysis of GmRUN1. (A) Domain architecture of GmRUN1. Yellow, green, and blue boxes indicate TIR, NBS, and LRR domains, respectively. TN: TIR-NBS, NL: NBS-LRR, TNL: TIR-NBS-LRR. (B,D) Transient expression assay of different domains for antiviral activity. N. benthamiana leaves were infiltrated with Agrobacterium GV3101 inocula (OD₆₀₀ = 1.0) carrying different recombinant vectors and co-infected with TMV-GFP (B) or SMV (D). GFP was visualized under hand-held UV lamp (Wavelength = 365 nm) at 5 dpi (days post infiltration). (C) Fluorescence quantification of truncated GmRUN1 transient expression assay. TMV-GFP intensity was analyzed by Gel-Pro analyzer software and normalized against positive control (GmRUN1 gDNA).