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. 2021 Oct 8;13(10):2030. doi: 10.3390/v13102030

Figure 2.

Figure 2

Efficient targeting of the 5′-UTR of SARS-CoV-2 by the designed siRNAs. The silencing activity of the designed siRNAs was examined in a dual-luciferase reporter assay. (A) Schematic representation of the reporter plasmid psiCheck2 SARS-CoV-2 5′-UTR. (B) Hela cells were co-transfected with 40 nM of the respective siRNA and 250 ng of the reporter plasmid. Proteins were extracted 48 h after transfection, the relative Ren/Luc activity was determined and the negative control (siCon) was set to 100%. The mean ± SEM of three independent experiments is shown. The statistical significance was determined by a univariate analysis of variance (one-way ANOVA); **** p < 0.0001.