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. 2021 Oct 8;13(10):2030. doi: 10.3390/v13102030

Figure 4.

Figure 4

Inhibition of SARS-CoV-2 alpha and SARS-CoV-1 replication by siCoV6. (A) The replication of SARS-CoV-2 alpha (BetaCoV/Germany/ChVir21652/2020) and SARS-CoV-1 (recombinant SARS-CoV-1, GenBank: AY310120.1) was investigated by quantitative RT-PCR in Vero E6 cells. Vero E6 cells were transfected with 100 nM siCoV6 or 100 nM siCon and infected 24 h after transfection with SARS-CoV-2 alpha or SARS-CoV-1 at a MOI of 0.01. Genome equivalents per mL (GE/mL) were determined by quantitative RT-PCR as described in Figure 3. The mean ± SEM of three independent experiments is shown. The statistical significance was determined by Student’s t-test; * p < 0.05. (B,C) Cell morphology observation. Cells were fixed 48 h after infection with SARS-CoV-2 alpha or SARS-CoV-1 and the cytopathic effect (CPE) of viral infection was documented by transmitted light microscopy. Representative data from three independent experiments are shown. Scale bar: 200 µm.