Skip to main content
. 2021 Oct 23;340:114–124. doi: 10.1016/j.jconrel.2021.10.023

Fig. 4.

Fig. 4

The in vivo delivery of the firefly reporter DNCA/CLD-mRNA in mice. Female BALB/c mice of 6–8 weeks old were inoculated with DNCA/CLD-mRNA-FLuc (mRNA-FLuc = 10 μg). (a) Representative bioluminescent images were obtained and analyzed at 6 h post inoculation of DNCA/CLD-mRNA-FLuc via different administration routes. (b) Representative bioluminescent images were obtained at 6 h post inoculation, and the photon flux intensities released from the commercially available in vivo jet-mRNA-FLuc-, Lipofectamine 2000-mRNA-FLuc-, and the DNCA/CLD-mRNA-FLuc-inoculated mice were analyzed after reaction with luciferase substrate. (c) Real-time monitor of the firefly luciferase expression in the DNCA/CLD-mRNA-FLuc-intramuscularly inoculated mice at indicated hours post inoculation (h.p.i). (d) Representative tissue biodistribution of DNCA/CLD-mRNA-FLuc in mice after intramuscular inoculation at 6 h, and mice treated with PBS were set as negative control. The intensity of photon flux was expressed by average radiance. Statistical significance was analyzed by a one-way ANOVA analysis followed by Dunnett's multiple comparisons (**** p < 0.0001, ** p < 0.01; ns: p > 0.05). Error bars were shown as the SEM of the quantified photon flux intensity released by the three tested mice.