Table 1.
RNA sequencing datasets of hemogenic endothelium and other hematopoietic tissues.
| Last Author, Year | Species | Type of Sequencing | Sorted Population(s) | Accession Number(s) |
Ref. |
|---|---|---|---|---|---|
| Zhang et al., 2015 | zebrafish | bulk RNAseq | flk1:mCherry+ (ECs), flk1:mCherry+/ runx1en:GFP+ (HE); and runx1en:GFP+(HSPCs) | N/A | [144] |
| Kartalaei et al., 2015 | mouse | bulk RNAseq | E10.5 AGM ECs(CD31+, cKit−,Ly6aGFP−), HE (CD31+, cKit−,Ly6aGFP+), HSCs(CD31+, cKit+,Ly6aGF+) and HPs( CD31+, cKit+,Ly6aGF+) | GSE63316 | [142] |
| Bonkhofer et al., 2019 | zebrafish | bulk RNAseq and ATACseq |
TgBAC(runx1P2:Citrine);Tg(kdrl: mCherry) to sort for HE, non-HE arterial ECs and venous ECs, including runx1 morphants |
GSE132259, GSE132258 |
[160] |
| Baron et al., 2018 | mouse | scRNA-seq | CD31+, cKit+ cells from E10 and E11 aorta after intra-aortic antibody staining, together with other aortic subfractions by surface markers | GSE112642 | [148] |
| Zeng et al., 2019 | human | scRNA-seq | Dissected AGM from ~30-day old human embryo (depleted of red blood cells) | GSE135202 | [149] |
| Yvernogeau et al., 2020 | zebrafish, mouse, chicken, human | Tomo-seq | zebrafish Tg(kdrl:mCherry/cd41:EGFP) at 28- and 40-hpf for HE/HSPC identification through trunk; E10.5 and E11.5 mouse trunk, E3 chicken embryo trunk, 35-day-old human embryo trunk | N/A | [147] |
| Chen et al., 2020 | mouse | scRNA-seq | Lin−, cKit+ cells with both Runx1-mKO2 and Ly6a-GFP transgenic reporters (HSCs) | GSE145638 | [150] |
| Zhu et al., 2020 | mouse | scRNA-seq | Purified EC, HE and intra-aortic cluster cells with surface markers and Runx1-GFP expression | GSE137117 | [163] |
| Oatley et al., 2020 | mouse | scRNA-seq | VE-cadherin+ cells from E10 AGM | E-MTAB-6987 | [164] |
| Kasper et al., 2020 | zebrafish | scRNA-seq | Dissected trunks from 27hpf wildtype and miR-223 mutants, sorted on Tg(kdrl:mCherry) ECs (also has miR-223 GFP+ population for clustering) | GSE135246 | [118] |
| Soto et al., 2021 | zebrafish | scRNA-seq | Tg(kdrl:EGFP) ECs at 30hpf from ezh1 wildtype, heterozygous and homozygous mutants | GSE173972 | [56] |
AGM: aorta-gonad-mesonephros, EC endothelial cell, HE hemogenic endothelium, HP hematopoietic progenitor, HSC hematopoietic stem cell, HSPC hematopoietic stem/progenitor cells.