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. 2021 Oct 15;22(20):11136. doi: 10.3390/ijms222011136

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Binding and aggregation characterization of different format anti-hNE antibodies. (a) V_H 1D1.43, Fab 1C10, V_H-Fc 1D1.43, and IgG1 1C10 binding to recombinant NE measured by ELISA. Experiments were performed in duplicate. (b) Aggregation evaluation of V_H 1D1, V_H 1D1.43, and V_H-Fc 1D1.43 measured by SEC. (c) Aggregation evaluation of Fab 1C10 and IgG1 measured by SEC. (d) Aggregation evaluation of V_H and Fab measured by DLS. Antibodies were evaluated at a concentration of 1 mg/mL. (e,f) Kinetics of V_H-Fc 1D1.43 (e) and IgG1 1C10 (f) binding to recombinant NE measured by BLItz. Values were reported as the mean ± SD.