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Specificity of VH/VH-Fc and Fab/IgG1 with recombinant human NE. (a,b) Binding of Fab 1C10 (a) and VH 1D1.43 (b) to rhNE, rhMPO, and PR3 measured by ELISA. (c,d) Binding of IgG1 1C10 (c) and VH-Fc 1D1.43 (d) to rhNE, rhMPO, BSA, and PR3 measured by ELISA. rhNE, rhMPO, BSA, and PR3 were coated in 96-well microplates at 50 ng/well. Experiments were performed in duplicate. Values were reported as the mean ± SD.
