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. 2021 Oct 8;11(10):1009. doi: 10.3390/jpm11101009

Figure 6.

Figure 6

PRMT1 regulates c-Myc downstream genes and homologous recombination (HR) genes in TNBC cells. (A) Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of MYC-targeted gene expression levels in PRMT1-knockdown MDA-MB-231 and BT549 cells. Knockdown efficacies of PRMT1 shRNAs in MDA-MB-231 and BT549 cells are shown. (B) RT-qPCR analysis of HR gene expression levels in PRMT1-knockdown MDA-MB-231 and BT549 cells. (C and D) MTT assay and colony formation assay showing that c-Myc overexpression rescued PRMT1 silencing–mediated growth inhibition in response to olaparib in MDA-MB-231 and BT549 cells. Quantification of clonogenic formation was carried out using Image J software. Data were obtained from three biological replicates. Unpaired t tests were performed to compare expression levels in two groups. * p < 0.05, ** p < 0.01, *** p < 0.001 were considered statistically significant.