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Evolutionary events related to genome enlargement in the exterior branch. Average gene family of (a) gain and (b) expansion (increasing paralog). (c,d). Gene family gained per branch among free-living Nostoc (FL), symbiotic Nostoc (SN), and non-Nostoc species (NN). Asterisks indicate significant differences based on Wilcoxon test (* p < 0.05 *** p < 0.001).
