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. 2021 Oct 19;22(20):11293. doi: 10.3390/ijms222011293

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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HA and FLAG tagging of coELTD1. (A) Western blot of HUVEC cells transduced with control versus HA-tagged coELTD1 expressing lentivirus probed with anti-ELTD1 and anti-HA tag antibodies. (B,C) Matrigel assay of control versus HA-tagged coELTD1 expressing HUVEC with quantification of network formation. (D) Fluorescent staining of VE-Cadherin and HA-tagged coELTD1 in control and HA-coELTD1 expressing HUVEC cells at 120 h. Images were taken on a Zeiss LSM 880 Confocal Microscope (Zeiss) at 63× magnification. *** p < 0.0005.