Figure 3.

Pretreatment of Lenti-S or host cells with GA on Lenti-S mediated luciferase gene delivery. (A) Diagram showing Lenti-S or host cells were treated (Tx) with 3 mM GA prior to the infection assay. Both Lenti-S and host cells were untreated or treated with 3 mM GA for 1 h. At the end, the GA-treated Lenti-S was 1:30 diluted and used to infect untreated host cells (final concentration of GA in the medium was approximately 0.1 mM). In parallel, the medium of the GA-treated cells was replaced with fresh medium without GA followed by infection of untreated Lenti-S. Luciferase activity was determined 24 h later. (B) GA effect on Lenti-S (Virus Tx) and on host cells (Cells Tx). Luciferase activity was expressed as a percentage of untreated controls. Data are mean ± SD of duplicate wells from 2 experiments. ns, no significance; **, p < 0.01 by Student’s t test.