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TEM images of CAV1 reconstituted in DPPC/CHOL (molar ratio, 1:1) liposomes. The protein-to-lipid weight ratios was 1:20. Small (A,B) and large (C) CAV1-induced buds are observed intact or ruptured (labeled by dotted arrows); solid arrows indicate membrane discontinuities at ruptured buds. CAV1-membrane aggregates labeled (D) and unlabeled (E,F) with Ni-NTA nanogold. In (E,F) well-defined buds are observed (labeled by dotted arrows) around very dense areas (labeled by solid arrows).
