. 2021 Oct 15;11(10):1093. doi: 10.3390/life11101093

Table 1.

Primers information for 5-locus DNA sequences.

Name		Length	Direction	Sequence 5′-3′	Optimised PCR Protocols	References
ITS	ITS5	22	forward	GGAAGTAAAAGTCGTAACAAGG	(95 °C: 30 s, 51 °C: 50 s, 72 °C: 45 s) × 33 cycles	[16]
ITS	ITS4	20	reverse	TCCTCCGCTTATTGATATGC	(95 °C: 30 s, 51 °C: 50 s, 72 °C: 45 s) × 33 cycles	[16]
LSU	LROR	17	forward	ACCCGCTGAACTTAAGC	(94 °C: 30 s, 51 °C: 1 min, 72 °C: 2 min) × 33 cycles	[17]
LSU	LR5	17	reverse	TCCTGAGGGAAACTTCG	(94 °C: 30 s, 51 °C: 1 min, 72 °C: 2 min) × 33 cycles	[17]
RPB1	CRPB1	20	forward	CAYCCWGGYTTYATCAAGAA	(94 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 33 cycles	[18]
RPB1	RPB1Cr	23	reverse	CCNGCDATNTCRTTRTCCATRTA	(94 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 33 cycles	[18]
RPB2	RPB2-5F3	20	forward	GACGACCGTGATCACTTTGG	(94 °C: 30 s, 54 °C: 40 s, 72 °C: 1 min 20 s) × 33 cycles	[19]
RPB2	RPB2-7Cr2	20	reverse	CCCATGGCCTGTTTGCCCAT	(94 °C: 30 s, 54 °C: 40 s, 72 °C: 1 min 20 s) × 33 cycles	[19]
TEF	983F	23	forward	GCYCCYGGHCAYCGTGAYTTYAT	(94 °C: 30 s, 58 °C: 1 min 20 s, 72 °C: 1 min) × 33 cycles	[19]
TEF	2218R	23	reverse	ATGACACCRACRGCRACRGTYTG	(94 °C: 30 s, 58 °C: 1 min 20 s, 72 °C: 1 min) × 33 cycles	[19]