Table 2.
Pathological results based on microscopic observation and quantitative scoring of HE and IHC staining lung samples, and percentage of lung-affected area.
| Group | Pig ID | Euthanasia Day | Lung-Affected Area (%) | Histopathological Scoring | Immunohistochemistry for SIV |
|---|---|---|---|---|---|
| Vaccinated Group | 1 | 2 dpi | 3.28 | 2 | ++ |
| 2 | 9.33 | 3 | +++ | ||
| 3 | 28.64 | 3 | ++ | ||
| 4 | 5 dpi | 13.22 | 2 | - | |
| 5 | 27.23 | 3 | - | ||
| 6 | 7.23 | 0.5 | - | ||
| 7 | 10 dpi | 8.57 | 1.5 | - | |
| 8 | 9.76 | 0.5 | - | ||
| mean | 13.41 | ||||
| Nonvaccinated Group | 9 | 2 dpi | 29.52 | 3 | +++ |
| 10 | 33.65 | 1.5 | + | ||
| 11 | 65.72 | 3 | + | ||
| 12 | 5 dpi | 24.5 | 2 | + | |
| 13 | 26.43 | 3 | + | ||
| 14 | 35.06 | 3 | + | ||
| 15 | 10 dpi | 16.36 | 1 | - | |
| mean | 33.03 |
Histopathology scoring: absence (0), few isolated (0.5), localized cluster (1), several (1.5–2), severely several (2.5), and many (3) airways affected. Moreover, minimal (1.5), mild (2) interstitial infiltrate, and plus moderate interstitial and alveolar infiltrates (2.5–3). SIV immunohistochemical scoring: absence (-), low (+), scattered (++), moderate (+++), and abundant (++++) amount of immunoreactivity.