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. 2021 Oct 14;22(20):11085. doi: 10.3390/ijms222011085

Figure 1.

Figure 1

The expression of KDM7A and DGAT2 is increased in FFAs-treated HepG2 and AML12 cells and in the liver of HFD-fed obese mice. (A,B) HepG2 and AML-12 cells were incubated with 1 mM FFAs for 24 h. TG levels were measured by a TG assay kit. Protein levels of KDM7A and DGAT2 were measured by Western blotting. mRNA levels of KDM7A and DGAT2 were measured by qPCR. Data are presented as the mean ± SD from three independent experiments. ** p < 0.01, *** p < 0.001 vs. untreated control. (C) C57BL/6 mice were fed a normal diet (ND) or high fat diet (HFD) for 16 weeks. Hepatic TG levels were measured by a TG assay kit. Protein levels of KDM7A and DGAT2 were assayed by Western blotting. Representative Western blot analysis of KDM7A and DGAT2 is shown. mRNA level of KDM7A was measured by qPCR. Data are presented as the mean ± SD from 6 mice per group. * p < 0.05, ** p < 0.01 vs. ND mice.