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. 2021 Oct 14;22(20):11085. doi: 10.3390/ijms222011085

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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KDM7A knockdown decreases DGAT2 expression and TG level in AML12 cells. AML12 cells were infected with Ad-GFP shRNA or Ad-KDM7A shRNA. (A) Protein levels of KDM7A, H3K9me, H3K27me2 and DGAT2 were measured by Western blotting. (B) mRNA levels of KDM7A and DGAT2 were measured by qPCR. (C) Intracellular TG levels were measured using a TG assay kit. Data are presented as the mean ± SD from three independent experiments. * p < 0.05 vs. control (Ad-GFP shRNA). (D) AML12 cells were infected with Ad-GFP shRNA or Ad-KDM7A shRNA and then incubated with 1 mM FFAs for an additional 24 h. Intracellular TG levels were measured using a TG assay kit. Data are presented as the mean ± SD from three independent experiments. ** p < 0.01 vs. control (Ad-GFP shRNA). ^# p < 0.05 vs. FFAs-treated Ad-GFP shRNA.