Table 3.

WNV surveillance plan (sampling and testing) in 2018.

	Lower-Risk Districts											Higher-Risk Districts
Testing of sentinel horses for WNV IgM antibodies (ELISA)
Sentinel horses	‑up to 20 horses ‑minimum of 5 localities per district ‑testing on four occasions (once per month in June, July, August, and September)											‑up to 40 horses ‑minimum of 8 localities per district ‑testing on four occasions (once per month in June, July, August, and September)
RT-qPCR detection of WNV in natural reservoirs and vectors
Wild birds	‑up to 20 dead wild birds found from May through October											‑up to 30 found dead throughout the year, or ‑up to 30 samples of purposely shot or live-trapped susceptible bird species during the period May–October
Mosquitoes (Culex pipiens)	‑collection at 2–3 week intervals ‑5 localities per district ‑4 samplings from June to August (2 samplings in July)											‑collection at 2–3 week intervals ‑10 localities per district ‑4 samplings from June to August (2 samplings in July)
Sampling strategy
Sampling distribution	Jan	Feb	Mar	Apr	May	Jun	Jul	Aug	Sep	Oct	Nov	Dec	Jan	Feb	Mar	Apr	May	Jun	Jul	Aug	Sep	Oct	Nov	Dec
Horses
Mosquitoes
Wild birds

The different colours only representing/highlighting horses, mosquitoes or wild birds.