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. 2021 Oct 11;13(10):1656. doi: 10.3390/pharmaceutics13101656

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The relative catalytic activity of serine hydrolases in HlungS9. The relative activity was profiled by the enrichment ratio after 1-min and 12-min incubations with the desthiobiotin-FP probe. The enrich ratio of a given hydrolase was calculated by dividing its abundance in the probe treated samples by its abundance in the control samples (without a probe, 1% DMSO). Bars show mean values (±S.D.) of three independent experiments (n = 3), except for LYPLA2 in the 1-min enrichment profile (n = 2). Values below the dashed line (enrichment ratio = 1) indicate no enrichment. * p < 0.05 and ** p < 0.01 indicate statistically significant difference compared with the 1-min incubations (Student’s t-test).