Table 1.
Meta-analysis of the protein abundance of CES1 and CatA in human respiratory and hepatic tissues and cells.
| Enzyme Resource | Study Reference | Sample Size | Assay Method and Quantification Type | Abundance | Abundance Ratio a CatA: CES1 |
CatA Contribution to Hydrolysis b | |
|---|---|---|---|---|---|---|---|
| CatA | CES1 | ||||||
| Pooled HLungS9 | [16] | 4 donors, triplicate measurement | Label-free DIA MS-based proteomics, absolute quantification | 0.130 ± 0.006 µg/mg total protein | 1.702 ± 0.063 µg/mg total protein | 1:13 | 97% |
| Pooled HLS9 | 200 donors, triplicate measurement | 0.071 ± 0.004 µg/mg total protein | 19.244 ± 0.083 µg/mg total protein | 1:271 | 64% | ||
| HLung S9 | [13] | n = 3 | Western blot analysis | 0.220 ± 0.004 µg/mg total protein | 2.1 ± 0.9 µg/mg total protein | 1:10 | 98% |
| HLS9 | n = 3 | 0.23 ± 0.11 µg/mg total protein | 34 ± 1 µg/mg total protein | 1:148 | 76% | ||
| Primary human NHBE cells | n = 3 | 0.33 ± 0.10 µg/mg total protein | <0.1µg/mg total protein | >3.3:1 | >99% | ||
| Primary human TBEC cells | [27] | n = 4 | Label-free DDA MS-based proteomics, absolute quantification | 0.056 ± 0.013 µg/mg total protein | 0.019 ± 0.017 µg/mg total protein | 3:1 | >99% |
| Primary human hepatocytes | [28] | n = 7 | 0.059 ± 0.014 µg/mg total protein | 13.7 ± 3.0 µg/mg total protein | 1:232 | 67% | |
| Lung tissue autopsy from COVID-19 patients | [26] | n = 30 | Labeled DDA MS-based proteomics, relative quantification | 1.501 ± 0.878 | 0.249 ± 0.069 | - | - |
| Liver tissue autopsy from COVID-19 patients | n = 24 | 1.308 ± 0.300 | 3.465 ± 1.078 | - | - | ||
| A549 cells (human pneumocyte type II carcinoma cells) | [29] | n = 3 | Labeled DDA MS-based proteomics, relative quantification | 0.765 | 0.797 | - | - |
| Huh-7 cells (human hepatocellular carcinoma cells) | n = 3 | 1.11 | 13.96 | - | - | ||
a For relative abundance data, it is suitable to compare the abundance of same protein between cells/tissues, but not rational to compare different proteins in the same sample. b CatA contribution was calculated by dividing the CatA contribution by the summation of CatA and CES1 contrition. The enzyme contribution was estimated by timing the enzyme activity with its abundance. NHBE: normal human bronchial epithelial cells; TBEC: tracheal bronchial epithelial cells.