Figure 2.

Western blot analysis of human EV-enriched serum preparations by using different procedures (UC, ultracentrifugation, SEC3, fractions 2–4 from SEC, SEC8 fractions 7–9 from SEC, INV, Total isolation solution from Invitrogen, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, GAG, Exo-GAG precipitation solution from Nasabiotech, A Coruña, Spain). * In the case of the sample INV, it was not possible to assay the sample against IgG, due to overload of protein, as shown in the small picture of the gel at the right corner.