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. 2021 Oct 1;10(10):2089. doi: 10.3390/plants10102089

Table 1.

Sequences and annealing temperatures of primers used for the RAPD and ISSR analysis of L. schweinfurthii.

Primer Name Sequence Annealing Temperature (°C)
RAPD primers OPA-10 5′-GTGATCGCAG-3′ 40.5
OPAJ-01 5′-ACGGGTCAGA-3′ 43
OPAK-06 5′-TCACGTCCCT-3′ 42
OPAK-20 5′-TGATGGCGTC-3′ 41
OPAQ-20 5′-GTGAACGCTC-3′ 40.5
OPB-18 5′-CCACAGCAGT-3′ 42
OPR-09 5′-TGAGCACGAG-3′ 42
ISSR primers HB11 5′-GTGTGTGTGTGTCC-3′ 54
HB12 5′-CACCACCACGC-3′ 50.9
HB13 5′- GAGGAGGAGGC-3′ 48
HB14 5′-CTCCTCCTCGC-3′ 48
HB15 5′-GTGGTGGTGGC-3′ 50.9