TABLE 2.
Effect of the presence of high concentrations of different contaminantsa on the immunomagnetic isolation of S. suis serotype 2 using PAb IgG-coated beads
| Initial inoculum size (no. of S. suis serotype 2 bacteria) | No. of S. suis serotype 2 CFU/ml recovered
|
||||
|---|---|---|---|---|---|
| Without contaminantsb | In presence of S. suis serotype:
|
||||
| 1/2 | 3 | 7 | 8 | ||
| 4.0 × 105 | (1.6 ± 0.4) × 105 | (1.9 ± 0.5) × 105 | (1.9 ± 0.1) × 105 | (2.1 ± 0.1) × 105 | (1.6 ± 0.4) × 105 |
| 4.0 × 104 | (3.0 ± 2.0) × 104 | (2.1 ± 1.3) × 104 | (2.1 ± 0.8) × 104 | (3.2 ± 1.8) × 104 | (2.6 ± 0.7) × 104 |
| 4.0 × 103 | (3.0 ± 1.0) × 103 | (1.4 ± 0.4) × 103 | (3.0 ± 1.8) × 103 | (2.0 ± 0.3) × 103 | (2.9 ± 1.3) × 103 |
| 4.0 × 102 | (3.6 ± 1.8) × 102 | (2.5 ± 1.2) × 102 | (1.7 ± 0.6) × 102 | (2.2 ± 1.2) × 102 | (1.5 ± 0.4) × 102 |
| 4.0 × 101 | (1.7 ± 1.2) × 101 | (2.1 ± 1.4) × 101 | (2.0 ± 0.5) × 101 | (2.3 ± 1.5) × 101 | (3.3 ± 2.8) × 101 |
a
At 105 to 106 CFU/ml.
b
Bacteria different from S. suis serotype 2. Not significantly different from recovery in the presence of S. suis serotype 1/2, 3, 7, or 8 (P > 0.1, Student’s unpaired t test).