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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
A schematic diagram shows the multiplexability of the CRISPR/dCas9 system. Enhancement of transcriptional regulation, simultaneous targeting of different genes and simultaneous targeted binding at multiple loci are possible using multiple sgRNAs.