Table 1.
Modification of CRISPR/dCas9 components for gene regulation in plant cells.
| CRISPR/dCas9 | Modification | Plant Species | Target Gene | Repression (%)/Activation (Fold-Change) | References |
|---|---|---|---|---|---|
| Transcriptional suppression | dCas9-SRDX | Nicotiana benthamiana | pNOS::LUC reporter | 33 | [95] |
| dCas9-BRD | N. benthamiana | pNOS::LUC reporter | 60 | [109] | |
| dCas9-3xSRDX | Arabidopsis | CFTS64 | 60 | [110] | |
| dCas9-TALE-SRDX | Arabidopsis | RD29-LUC (1) | - | [111] | |
| dLbCpf1-SRDX | Arabidopsis | miR159B | 90 | [112] | |
| dAsCpf1-SRDX | Arabidopsis | miR159B | 90 | [112] | |
| Transcriptional activation | dCas9VP64 | Oryza sativa | Os03g01240 | 2.0 | [113] |
| dCas9-TV | O. sativa | OsER1 | 62.0 | [108] | |
| dCas9VP64+MS2-VP64 | O. sativa | Os04g39780 | 4.0 | [113] | |
| dCas9-VP64 | Arabidopsis | pWRKY::luciferase | 6.7 | [108] | |
| dCas9-MCP-TV | Arabidopsis | AtWRKY | 11.7 | [108] | |
| dCas9-SunTag | Arabidopsis | AtCLAVATA3 | 100.0 | [114] | |
| dCas9:SunTag-EDLL | N. benthamiana | pNOS::luciferase | 3.0 | [115] | |
| dCas9-VP64 | N. benthamiana | pNOS::luciferase | 3.0 | [95] | |
| dCas9-EDLL | N. benthamiana | NbPDS | 3.4 | [95] |