Table 2.
Application of CRISPR/dCas9 in plants.
| Application | Plant Species | Modification | Reference |
|---|---|---|---|
| Live cell chromatin imaging | N. benthamiana | dCas9-eGFP | [127] |
| dCas9-FP | [128] | ||
| dCas9-MS2-mRuby2 | [129] | ||
| Transcriptional regulation | Arabidopsis | dCas9-MCP-TV | [108] |
| dCas9-3xSRDX | [110] | ||
| dAsCpf1-SRDX | [112] | ||
| O. sativa | LUC/dCas9-TV | [123] | |
| dCas9VP64+ MS2-VP64 | [113] | ||
| dCas9-TV-6 × His | [108] | ||
| N. benthamiana | dCas9-VP128 | [95] | |
| dCas9-EDLL | [95] | ||
| Epigenetic manipulation | Arabidopsis | dCas9-MS2 | [121] |
| dCas9-TET1cd | [130,131] | ||
| dCas9-SunTag | [114] | ||
| Chromatin topology | Arabidopsis | dCas9-PYL1 | [132] |
| dCas9-ABI1 | [89] |