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Representative images of DAB-stained CB1-expressing neurons in the periventricular nucleus (PVN) of cows after intraperitoneal administration with NaCl (n = 7), AEA (n = 7) or 2-AG (n = 6) for 9 days. CB1 immunoreactivity was visualized with DAB (arbitrary units, AU) and nuclei were stained with hemalaun (blue). Scale bar within images indicates 20 µm. CB1 intensities (a) are shown as LSM ± SE with F(2,15) = 0.15, p = 0.86 (ANOVA).
