Table 1.
Relative mRNA abundances (LSM ± SE) of orexigenic neuropeptides and genes related to the ECS of the arcuate nucleus (ARC) and paraventricular nucleus (PVN). Samples were obtained from cows treated with NaCl (n = 7), AEA (n = 7) or 2-AG (n = 6) for 9 days.
| Gene | NaCl | AEA | 2-AG | ANOVA |
|---|---|---|---|---|
| ARC | ||||
| AGRP | 1.72 ± 0.46 | 0.55 ± 0.04 | 2.03 ± 0.87 | F(2,17) = 3.13, p = 0.07 |
| CNR1 | 1.03 ± 0.16 | 1.06 ± 0.17 | 1.11 ± 0.14 | F(2,17) = 0.06, p = 0.95 |
| DAGLA | 1.00 ± 0.09 | 0.98 ± 0.09 | 1.07 ± 0.07 | F(2,17) = 0.29, p = 0.75 |
| FAAH | 1.07 ± 0.18 | 0.98 ± 0.10 | 1.12 ± 0.07 | F(2,17) = 0.31, p = 0.73 |
| NAPELD | 1.05 ± 0.03 | 0.95 ± 0.11 | 1.07 ± 0.11 | F(2,17) = 0.53, p = 0.60 |
| NPY * | 1.84 ± 0.70 | 0.42 ± 0.11 | 1.91 ± 0.48 | F(2,15) = 2.81, p = 0.09 |
| PLAAT5 * | 1.16 ± 0.45 | 1.43 ± 0.42 | 1.05 ± 0.23 | F(2,14) = 0.39, p = 0.68 |
| PVN | ||||
| AGRP | 1.17 ± 0.17 | 1.15 ± 0.38 | 1.48 ± 0.47 | F(2,17) = 0.24, p = 0.79 |
| CNR1 | 0.91 ± 0.07 | 1.07 ± 0.24 | 1.37 ± 0.24 | F(2,17) = 1.12, p = 0.35 |
| DAGLA | 0.99 ± 0.06 | 1.06 ± 0.10 | 1.00 ± 0.14 | F(2,17) = 0.16, p = 0.85 |
| FAAH | 0.99 ± 0.10 | 1.00 ± 0.07 | 1.08 ± 0.09 | F(2,17) = 0.29, p = 0.75 |
| NAPELD | 1.01 ± 0.09 | 1.06 ± 0.09 | 1.00 ± 0.08 | F(2,17) = 0.12, p = 0.89 |
| PLAAT5 | 1.38 ± 0.24 | 1.26 ± 0.46 | 1.65 ± 0.66 | F(2,17) = 0.18, p = 0.84 |
* Because of the limited amount of ARC mRNA, evaluable results were limited to NaCl (n = 7), AEA (n = 7), 2-AG (n = 4) and NaCl (n = 5), AEA (n = 6), 2-AG (n = 6), respectively. AGRP, agouti-related neuropeptide; CNR1, cannabinoid receptor 1; DAGLA, diacylglycerol lipase alpha; FAAH, fatty acid amide hydrolase; NAPEPLD, N-acyl phosphatidylethanolamine phospholipase D; NPY, neuropeptide Y; PLAAT5, phospholipase A and acyltransferase 5.