Figure 3.

Kinetic analyses of ⁰NCp7, NCp7 and ²NCp7 binding to SL3-RNA, determined by SPR. Representative SPR kinetic curves and fits for (A) NCp7, (B) ⁰NCp7 and (C) ²NCp7 binding to SL3-RNA. The amount of SL3-RNA immobilized on the chip surface was 50 RU (A, C) and 100 RU (B), respectively. The concentrations of NCp7 and ²NCp7 are 0.625, 1.25, 2.5, 5 and 10 nM, whereas the concentrations of ⁰NCp7 are 2.5, 5, 10, 20 and 40 nM. Experimental and fitted curves are colored and black, respectively. (D) SPR parameters of ⁰NCp7, NCp7 and ²NCp7 binding to SL3-RNA, generated from two independent experiments, with standard deviations in brackets. Chi-square values are also shown, which describe the deviation between the experimental and fitted curves.