Table 1.
Karber’s method for the determination of the LD50 of DBT and DBT–CSNPs in rats.
| Groups | Dose (mg/kg) | Dose Difference (a) | No. of Rats | LD50 of DBT | LD50 of DBT–CSNPs | ||||
|---|---|---|---|---|---|---|---|---|---|
| No. of Dead | Mean Mortality (b) | Product (a × b) | No. of Dead | Mean Mortality (b) | Product (a × b) | ||||
| 1 | 200 | - | 4 | 0 | - | - | 0 | - | - |
| 2 | 400 | 200 | 4 | 1 | 0.5 | 100 | 0 | - | - |
| 3 | 800 | 400 | 4 | 1 | 1 | 400 | 1 | 0.5 | 200 |
| 4 | 1200 | 400 | 4 | 2 | 1.5 | 600 | 1 | 1 | 400 |
| 5 | 2000 | 800 | 4 | 3 | 2.5 | 2000 | 2 | 1.5 | 1200 |
| 6 | 3000 | 1000 | 4 | 4 | 3.5 | 3500 | 4 | 3 | 3000 |
| Sum of product | 6600 | 4800 | |||||||
| LD50 | LD50 = 3000 ‒ (6600/4) = 1350 mg/kg |
LD50 = 3000 − (4800/4) = 1800 mg/kg |
|||||||