Table 2.
Panels of SARS-CoV-2 positive (N = 106) and negative (N = 26) human and veterinary samples tested for validation of the developed assay.
| Panel | Mutation Identification Method | No. | Variant | Fluorescence | |||
|---|---|---|---|---|---|---|---|
| F | H | T | C | ||||
| Negative | N/A | 20 | N/A | - | - | - | - |
| Positive (A) | Obtained prior to the emergence of the target mutations | 20 | WT | + | - | - | - |
| ViroBOAR Spike 1.0 RT-PCR Kit | 4 | WT | + | - | - | - | |
| 21 | Alpha | + | - | - | + | ||
| 1 | Beta | - | - | + | + | ||
| SNPsig SARS-CoV-2 (EscapePLEX) | 14 | Delta | + | + | - | - | |
| Positive (B) | NGS (Illumina MiSeq) | 2 | Alpha | + | - | - | + |
| 2 | Beta | - | - | + | + | ||
| 36 | Delta | + | + | - | - | ||
| 1 | B.1.1.318 | - | - | + | - | ||
| Negative (Vet) | N/A | 3 cats, 3 minks | N/A | - | - | - | - |
| Positive (Vet) | NGS (Ion Torrent GeneStudio S5) | 2 cats | WT | + | - | - | - |
| NGS (Illumina MiSeq) | 3 minks | WT | + | - | - | - | |
+: fluorescence detection in the respective channel; -: absence of fluorescence in the respective channel; F: FAM; H: HEX; T: Texas Red; C: Cy5; N/A: not applicable; NGS: next-generation sequencing; WT: wild type.