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Cellular uptake of complexes with optimal concentrations of AuNP after 3 and 24 hours of incubation in (a) HeLa, (b) HL-60, and (c) CEM-SS. Cells were treated with 100 µg/mL for AuNP13; 25 µg/mL for AuNP14; 10 µg/mL for AuNP15. Used siRNA is rndRNA-FAM (100 nM). Data obtained based on fluorescence intensity from FAM-labeled RNA by flow cytometry. Statistical significance between 3 and 24 h (at * p < 0.001) was estimated by the post-hoc Newman–Keuls test.
