Figure 3.

Effect of polyphenolic extracts from SCG on the (a) SOD and (b) CAT activities in C. viridis brain cells under H₂O₂-induced oxidative stress. Cells were pretreated with the SCG extracts (115–460 µg/mL) for 24 h and then incubated in the presence of 100 µM H₂O₂ for 1 h. C: control, non-treated cells; C +: cells treated with 100 µM H₂O₂ alone; SCG: cells pretreated with the spent coffee ground polyphenolic extracts at 115, 230 and 460 µg/mL, and treated with 100 µM H₂O₂. Data are presented as means ± SD (n = 3). Different letters (a, b, ab) indicate significant differences between the treatments (p < 0.05).