Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HKU10 spike-mediated pseudovirus entry. Ro-BtCoV HKU10, LA7560, and MERS-CoV spike-mediated pseudovirus were produced to screen their cell tropism. For Trypsin treatment, pseudoviruses were incubated with 50 μg/mL TPCK-treated trypsin at room temperature for 10 min, and then add 50 μg/mL soybean trypsin inhibitor to stop the reactions. Trypsin treated or untreated pseudoviruses were added to cells and incubated. Then, 48 h post infection, cells were lysed and luciferase were detected.
