Table 2.
Analytical performance of ADH1B targeted proteomics assay.
| Performance Parameter | Result |
|---|---|
| Matrix effects and interferences (bias) | |
| Pool of plasma from five healthy donors | −10.0% |
| Pool of serum from six healthy donors | −14.0% |
| Hemolytic serum (hemoglobin 500 mg/dL) | −24.0% |
| Lipemic serum (triglycerides 200 mg/dL) | −14.0% |
| Lipemic serum (triglycerides 500 mg/dL) | 0.0% |
| Serum with medium bilirubin (82 µmol/L) | −14.0% |
| Serum with high bilirubin (326 µmol/L) | −10.0% |
| Calibration curve | |
| Range of tested concentrations | 1 to 384 µg/mL |
| Accuracy (trueness) | 120.0% |
| Linearity (R2) | 1.00 |
| LLOD | 2 µg/mL |
| LLOQ | 6 µg/mL |
| Technical precision at LLOQ (CV, n = 3) | 8.0% |
| Repeatability and reproducibility | |
| Intraday (CV, n = 5) | 3.2% |
| Interday (CV, n = 5) | 4.2% |
| Sample handling stability (bias) | |
| Sample storage for 4 h at room temperature | −2.2% |
| Sample storage 24 h at 4 °C | 1.8% |
| Two freeze–thaw cycles | 1.0% |
| Frozen sample storage (−20 °C) for 30 days | 1.4% |
| Analyte stability (bias) | |
| 3 months at −80 °C (n = 2 samples from ALI patients) | 19.8% |
| Peptide stability (bias) | |
| Sample storage for 4 h at room temperature | 3.7% |
| Sample storage 24 h at 4 °C | 3.7% |
| Two freeze–thaw cycles | 0.0% |
| Frozen sample storage (−80 °C) for >30 days | 7.4% |