Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 30;10(10):2064. doi: 10.3390/plants10102064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Purification of recombinant OsCHIs expressed in E. coli. The His-tagged recombinant OsCHI1, OsCHI3, OsCHI6, and OsCHI7 proteins were expressed as a soluble form in E. coli. The recombinant OsCHI3, OsCHI6, and OsCHI7 proteins were purified with Ni²⁺-affinity chromatography. OsCHI1 was purified with Ni²⁺-affinity chromatography followed by cation-exchange chromatography. M, Molecular weight marker.