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qRT-PCR analysis of OsCHI gene expression in rice seedlings and different tissues. A ubiquitin gene (OsUBQ5) was amplified using specific primers and was used as an internal control. Expression level of each OsCHI gene is presented as the relative expression compared to the OsUBQ5 mRNA level. Asterisks indicate significant differences (* p < 0.05, ** p < 0.005, Tukey’s HSD-test). qRT-PCR analysis was performed on triplicate biological samples.
