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Kinetic parameters of recombinant OsCHI3 1.
| Substrate | KM (µM) | Vmax (nmol s−1 mg−1) | kcat (s−1) |
kcat/KM (M−1 s−1) |
|---|---|---|---|---|
| Naringenin chalcone | 11.60 ± 0.78 | 2662 ± 207.9 | 69.35 | 5.978 × 106 |
| Isoliquiritigenin | 50.95 ± 4.83 | 3.54 × 10−3 ± 1.25 × 10−4 | 9.214 × 10−5 | 1.809 |
1 The results represent the mean ± standard deviation of three independent experiments.
