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. 2021 Oct 4;14(10):1020. doi: 10.3390/ph14101020

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Compound-mediated changes in pAkt/Akt and pErk/Erk in PC3 cells. PC3 cells were treated with SAHA, Jazz90 and Jazz167 at 1 and 4 µM. Vehicle control cells (C) were treated with 0.5% DMSO. Cells were harvested 24 h after treatment. Representative Western blots for (a) pAkt, Akt and β-actin (loading control) and (b) pErk, Erk and β-tubulin (loading control). Densitometry of scanned Western blots for (c) pAkt and (d) pErk is shown. Bars represent the mean ± S.E.M. from 3 independent experiments, as shown by the data points. Data were analysed using a two-way ANOVA followed by a Bonferroni’s post-hoc test. *significantly different relative to the control, p < 0.05.